lysA [2019-08-07 09:48:16]

diaminopimelate decarboxylase

Locus: BSU_23380

Isoelectric point: 5.01

Molecular weight: 48.41 kDa

Protein length: 439 aa

Gene length: 1317 bp

Function: biosynthesis of lysine

Product: diaminopimelate decarboxylase

Essential: no

E.C.: 4.1.1.20

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of lysine/ threonine
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

Gene

Coordinates: 2,436,947 → 2,438,266

Phenotypes of a mutant

• auxotrophic for lysine PubMed
• reduced conjugation of ICEBs1 PubMed

The protein

Catalyzed reaction/ biological activity

• Meso-2,6-diaminoheptanedioate = L-lysine + CO₂ (according to Swiss-Prot)

Protein family

• Orn/Lys/Arg decarboxylase class-II family (single member, according to UniProt)

Cofactors

• PLP (according to UniProt)

Structure

• 1HKW (from Mycobacterium tuberculosis, 42% identity, 61% similarity) PubMed

Expression and Regulation

Operons

• Genes: spoVAA-spoVAB-spoVAC-spoVAD-spoVAEB-spoVAEA-spoVAF-lysA
  Description: PubMed

  Sigma factors

  • SigG: sigma factor, PubMed, in SigG regulon

  Regulatory mechanism

  • SpoVT: activation, PubMed, in SpoVT regulon

  Regulation

  • expressed late during sporulation in the forespore (SigG, SpoVT) PubMed

• Genes: lysA
  Description: PubMed

Biological materials

Mutant

• 1A615 ( lysA::erm), PubMed, available at BGSC
• BKE23380 (ΔlysA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATGTCATTCCCTCTTTCT, downstream forward: _UP4_TAAAAGAAAGCGCCGATTTT
• BKK23380 (ΔlysA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATGTCATTCCCTCTTTCT, downstream forward: _UP4_TAAAAGAAAGCGCCGATTTT

References

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